【原著論文】

1.       Yutaka Kirimoto, Noriko Yamano-Adachi, Yuichi Koga, Takeshi Omasa, Effect of co-overexpression of the cargo receptor ERGIC-53/MCFD2 on antibody production and intracellular IgG secretion in recombinant Chinese hamster ovary cells., J. Biosci. Bioeng., in press, 2022

2.       Sudarat Rodpan, Jiddah Nafiu Usman, Yuichi Koga, Nujarin Jongruja, Synergistic effect of Nisin with acetic and propionic acids inactivates Bacillus subtilis on meat and potato, Biocatalysis and Agricultural Biotechnology, 41, 102317, 2022

3.       Samy A., Yamano-Adachi N., Koga Y., Omasa T., Secretion of a low‐molecular‐weight species of endogenous GRP94 devoid of the KDEL motif during endoplasmic reticulum stress in Chinese hamster ovary cells., Traffic, 22(12), 425-438, 2021  

4.       Enatsu H., Okamoto N., Nomura Y., Onitsuka Y., Yamano-Adachi N., Koga Y., Omasa T., Production of monoclonal shark-derived immunoglobulin new antigen receptor antibodies using Chinese hamster ovary cell expression system., J. Biosci. Bioeng., 132(3) , 302-309, 2021   

5.       Uehara R., Dan N., Amesaka H., Yoshizawa T., Koga Y.., Kanaya S., Takano K., Matsumura H., Tanaka SI., Insertion loop‐mediated folding propagation governs efficient maturation of hyperthermophilic Tk-subtilisin at high temperatures., FEBS letters, 595(4), 452-461, 2021  

6.       Sakudo A., Imanishi Y., Hirata A., Koga Y., Shintani H., Effect of Nitrogen Gas Plasma Generated by a Fast-Pulsed Power Supply Using a Static Induction Thyristor on Scrapie Prion., Pathogens, 9(10), 819-829, 2020

7.       Hokao R, Matsumura H, Katsumi R, Angkawidjaja C, Takano K, Kanaya S, Koga Y*., Affinity shift of ATP upon glycerol binding to a glycerol kinase from the hyperthermophilic archaeon Thermococcus kodakarensis KOD1., J. Biosci. Bioeng., 129(6), 657-663, 2020

8.       Koga Y.*, Konishi K., Kobayashi A., Kanaya S., Takano K., Anaerobic glycerol-3-phosphate dehydrogenase complex from hyperthermophilic archaeon Thermococcus kodakarensis KOD1., J. Biosci. Bioeng., 127(6), 679-685, 2019

9.       Kaneyoshi K., Kuroda K., Uchiyama K., Onitsuka M., Yamano-Adachi N., Koga Y., Omasa T., Secretion analysis of intracellular "difficult-to-express" immunoglobulin G (IgG) in Chinese hamster ovary (CHO) cells., Cytotechnology, 71(1), 305-316, 2019

10.     Kaneyoshi K., Yamano N., Koga Y., Uchiyama K., Omasa T., Analysis of the immunoglobulin G (IgG) secretion efficiency in recombinant Chinese hamster ovary (CHO) cells by using Citrine-fusion IgG, Cytotechnology, 71(1), 193-207, 2019

11.     Kaneyoshi K., Uchiyama K., Onitsuka M., Yamano N., Koga Y., Omasa T., Analysis of intracellular IgG secretion in Chinese hamster ovary cells to improve IgG production., J. Biosci. Bioeng., 127(1), 107-113, 2019

12.     Murata D., Okano H., Angkawidjaja C., Akutsu M., Tanaka SI., Kitahara K., Yoshizawa T., Matsumura H., Kado Y., Mizohata E., Inoue T., Sano S., Koga Y., Kanaya S., Takano K., Structural basis for the Serratia marcescens lipase secretion system: crystal structures of the membrane fusion protein and nucleotide-binding domain, Biochemistry, 56(47), 6281-6291, 2017

13.     Hirata A., Sakudo A., Takano K., Kanaya S., Koga Y*., Effects of surfactant and a hyperthermostable protease on infectivity of scrapie-infected mouse brain homogenate, J. Biotech. Biomat., 5(3), 1000194(1- 4), 2015

14.     Okano H., Ozaki M., Kanaya E., Kim J. J., Angkawidjaja C., Koga Y., Kanaya S., Structure and stability of metagenome-derived glycoside hydrolase family 12 cellulase (LC-CelA) a homolog of Cel12A from Rhodothermus marinus, FEBS Open Bio., 4, 936-946, 2014

15.     You D. J., Jongruja N., Tannous E., Angkawidjaja C., Koga Y., Kanaya S., Structural basis for salt-dependent folding of ribonuclease H1 from halophilic archaeon Halobacterium sp. NRC-1, J. Struct. Biol., 187(2), 119-128, 2014

16.     Koga Y., Tanaka S., Sakudo A., Tobiume M., Aranishi M., Hirata A., Takano K., Ikuta K., Kanaya S., Proteolysis of abnormal prion protein with a thermostable protease from Thermococcus kodakarensis KOD1, Appl. Microbiol. Biotechnol., 98(5), 2113-2120, 2014

17.     Seino S., Watnabe K., Shikakura T., Michika A., Nakagawa T., Koga Y. and Yamamoto T. A., Enzyme immobilization on gold/Fe-oxide composite nanoparticles using a methionine tag, Coll. Surf. A: Physico. Eng. Asp., 459, 298-301, 2014

18.     Cheng M., Angkawidjaja C., Koga Y., Kanaya S., Calcium-independent opening of lid1 of a family I.3 lipase by a single Asp to Arg mutation at the calcium-binding site., Protein Eng. Des. Sel. 27(5), 169-176, 2014

19.     Seino S., Yoshida K., Shikakura T., Watnabe K., Koga Y., Nakagawa T. and Yamamoto T. A., Control the functionality of thermoresponsive enzymes immobilized onto magnetic nanoparticles by the application of alternating magnetic field, J. Magn. Soc. Jpn., 28, 98-101, 2014

20.     Sulaiman S., You D. J., Kanaya E., Koga Y., Kanaya S. Crystal structure and thermodynamic and kinetic stability of metagenome-derived LC-cutinase, Biochemistry, 53(11), 1858-1869, 2014

21.     Okada K., Angkawidjaja C., Koga Y., Kanaya S., Structural and mechanistic insights into the kynurenine aminotransferase-mediated excretion of kynurenic acid, J. Struct. Biol., 185(3), 257-266, 2014

22.     Uehara R., Angkawidjaja C., Koga Y., Kanaya S. Formation of the high-affinity calcium binding site in pro-subtilisin E with the insertion sequence IS1 of pro-Tk-subtilisin, Biochemistry, 52(50), 9080-9088, 2013

23.     Yuzaki K., Sanda Y., You D. J., Uehara R., Koga Y., Kanaya S., Increase in activation rate of Pro-Tk-subtilisin by a single nonpolar-to-polar amino acid substitution at the hydrophobic core of the propeptide domain, Protein Sci., 22(12), 1711-1721, 2013

24.     Permanasari E. D., Angkawidjaja C., Koga Y., Kanaya S., Role of N-terminal extension of Bacillus stearothermophilus RNase H2 and C-terminal extension of Thermotoga maritima RNase H2, FEBS J., 280(20), 5065-5079, 2013

25.     Takano K., Aoi A., Koga Y., Kanaya S., Evolvability of thermophilic proteins from archaea and bacteria. Biochemistry, 52(28), 4774-4780, 2013

26.     Nguyen T.N., You D.J., Matsumoto H., Kanaya E., Koga Y., Kanaya S., Crystal structure of metagenome-derived LC11-RNase H1 in complex with RNA/DNA hybrid, J. Struct. Biol., 182(2), 144-154, 2013

27.     Tadokoro T., Kazama H., Koga Y., Takano K., Kanaya S., Investigating the structural dependence of protein stabilization by amino acid substitution, Biochemistry, 52(16), 2839-2847, 2013

28.     Nguyen T. N., You D. J., Kanaya E., Koga Y., Kanaya S., Crystal structure of metagenome-derived LC9-RNase H1 with atypical DEDN active site motif, FEBS Lett., 587(9), 1418-1423, 2013

29.     Hirata A., Hori Y., Koga Y., Okada J., Sakudo A., Ikuta K., Kanaya S., Takano K., Enzymatic activity of a subtilisin homolog, Tk-SP, from Thermococcus kodakarensis in detergents and its ability to degrade the abnormal prion protein, BMC Biotechnol., 13, 19, 2013

30.     Uehara R., Ueda Y., You D. J., Koga Y., Kanaya S., Accelerated maturation of Tk-subtilisin by a Leu Pro mutation at the C-terminus of the propeptide, which reduces the binding of the propeptide to Tk-subtilisin, FEBS J., 280(4), 994-1006, 2013

31.     Okada J., Koga Y., Takano K., Kanaya S., Slow unfolding pathway of hyperthermophilic Tk-RNase H2 examined by pulse proteolysis using the stable protease Tk-subtilisin, Biochemistry, 51(45), 9178-9191, 2012

32.     Cheng M., Angkawidjaja C., Koga Y., Kanaya S., Requirement of lid2 for interfacial activation of a family I.3 lipase with unique two lid structures., FEBS J., 279(19), 3727-3737, 2012

33.     Uehara R., Tanaka S., Takano K., Koga Y., Kanaya S., Requirement of insertion sequence IS1 for thermal adaptation of Pro-Tk-subtilisin from hyperthermophilic archaeon, Extremophiles, 16(6), 841-851, 2012

34.     Angkawidjaja C., Koga Y., Takano K., Kanaya S., Structure and stability of a thermostable carboxylesterase from the thermoacidophilic archaeon Sulfolobus tokodaii, FEBS J., 279(17), 3071-3084, 2012

35.     Jongruja N., You D. J., Angkawidjaja C., Kanaya E., Koga Y., Kanaya S., Structure and characterization of RNase H3 from Aquifex aeolicus, FEBS J., 279(15), 2737-2753, 2012

36.     Okada K., Angkawidjaja C., Koga Y., Takano K., Kanaya S., Characteristic features of kynurenine aminotransferase allosterically regulated by -ketoglutarate in cooperation with kynurenine, PLoS One, 7(7), e40307, 2012

37.     Uehara R., Takeuchi Y., Tanaka S., Takano K., Koga Y., Kanaya S., Requirement of Ca(2+) ions for the hyperthermostability of Tk-subtilisin from Thermococcus kodakarensis, Biochemistry 51(26), 5369-5378, 2012

38.     Nguyen T. N., Angkawidjaja C., Kanaya E., Koga Y., Takano K., Kanaya S., Activity, stability, and structure of metagenome-derived LC11-RNase H1, a homolog of Sulfolobus tokodaii RNase H1, Protein Sci., 21(4):553-561

39.     Budiman C., Tadokoro T., Angkawidjaja C., Koga Y., Kanaya S., Role of polar and nonpolar residues at the active site for PPIase activity of FKBP22 from Shewanella sp. SIB1, FEBS J., 279 (6), 976-986, 2012

40.     Sulaiman S., Yamato S., Kanaya E., Kim J. J., Koga Y., Takano K., Kanaya S., Isolation of a novel cutinase homolog with polyethylene terephthalate-degrading activity from leaf-branch compost by using a metagenomic approach, Appl. Environ. Microbiol., 78(5):1556-1562, 2012

41.     Budiman C., Angkawidjaja C., Motoike H., Koga Y., Takano K., Kanaya S., Crystal structure of N-domain of FKBP22 from Shewanella sp. SIB1: Dimer dissociation by disruption of Val-Leu knot, Protein Sci., 20, 755-764, 2011

42.     Kim J. J., Kanaya E., Weon H. Y., Koga Y., Takano K., Dunfield P. F., Kwon S. W., Kanaya S., Flavobacterium compostarboris sp. nov., isolated from leaf-and-branch compost, and emended descriptions of Flavobacterium hercynium, Flavobacterium resistens and Flavobacterium johnsoniae, Int. J. Syst. Evol. Microbiol., 62(Pt 8):2018-2024, 2012

43.     Budiman C., Koga Y., Takano K., Kanaya S., FK506-binding protein 22 from a psychrophilic bacterium, a cold shock-inducible peptidyl prolyl isomerase with the ability to assist in protein folding, Int. J. Mol. Sci., 12, 5261-5284, 2012

44.     Miyashita S., Tadokoro T., Angkawidjaja C., You D. J., Koga Y., Takano K., Kanaya S., Identification of the substrate binding site in the N-terminal TBP-like domain of RNase H3, FEBS Lett., 585, 2313-2317, 2011

45.     Sinsereekul N., Foophow T., Yamanouchi M., Koga Y., Takano K., Kanaya S., An alternative mature form of subtilisin homologue, Tk-SP, from Thermococcus kodakaraensis identified in the presence of Ca2+, FEBS J., 278, 1901-1911, 2011

46.     Tanaka SI., Koga Y., Takano K., Kanaya S., Inhibition of chymotrypsin and subtilisin-like serine proteases with Tk-serpin from hyperthermophilic archaeon Thermococcus kodakaraensis, Biochim. Biophys. Acta, 14, 299-307, 2011

47.     Takano K., Okamoto T., Okada J., Tanaka S., Angkawidjaja C., Koga Y., Kanaya S., Protein stabilization tag: stabilization by fusion to the C-terminus of hyperthermophile Sulfolobus tokodaii RNase HI, PLoS ONE, 6, e16226, 2011

48.     Sato A., Yokotani A., Tadokoro T., Tanaka S., Angkawidjaja C., Koga Y., Takano K., Kanaya S., Crystal structure of stable protein, CutA1, from a psychrotrophic bacterium Shewanella sp. SIB1, J. Synchrotron Rad., 18, 6-10, 2011

49.     Kuwahara K., Angkawidjaja C., Koga Y., Takano K., Kanaya S., Importance of an extreme C-terminal motif of a family I.3 lipase for stability., Protein Eng. Des. Sel., 24, 411-418, 2011

50.     Iefuji N., Murai R., Maruyama M., Takahashi Y., Sugiyama S., Adachi H., Matsumura H., Murakami S., Inoue T., Mori Y., Koga Y., Takano K., Kanaya S., Laser-induced nucleation in protein crystallization: local increase in protein concentration induced by femtosecond laser irradiation, J. Cryst. Growth, 318, (1), 741-744, 2011

51.     Jongruja N., You D. J., Kanaya E., Koga Y., Takano K., Kanaya S., The N-terminal hybrid binding domain of RNase HI from Thermotoga maritima is important for substrate binding and Mg(2+) -dependent activity, FEBS J., 277, 4474-4489, 2010

52.     Kim J. J., Jin H. M., Lee H. J., Jeon C. O., Kanaya E., Koga Y., Takano K., Kanaya S., Flavobacterium banpakuense sp. nov. isolated from leaf-and-branch compost, Int. J. Syst. Evol. Microbiol., 61, 1595-1600. 2010

53.     Tanaka M., Chon H., Angkawidjaja C., Koga Y., Takano K., Kanaya S., Protein core adaptability: crystal structures of the cavity-filling variants of Escherichia coli RNase HI, Protein Pept. Lett., 17, 1163-1169, 2010

54.     Kanaya E., Sakabe T., Nguyen N. T., Koikeda S., Koga Y., Takano K., Kanaya S., Cloning of the RNase H genes from a metagenomic DNA library: identification of a new type 1 RNase H without a typical active-site motif, J. Appl. Microbiol., 109, 974-983, 2010

55.     Okada J., Okamoto T., Mukaiyama A., Tadokoro T., You D. J., Chon H., Koga Y., Takano K., Kanaya S., Evolution and thermodynamics of the slow unfolding of hyperstable monomeric proteins, BMC Evol. Biol., 10, 207, 2010

56.     Foophow T., Tanaka S., Angkawidjaja C., Koga Y., Takano K., Kanaya S., Crystal structure of a subtilisin homologue, Tk-SP, from Thermococcus kodakaraensis: requirement of a C-terminal beta-jelly roll domain for hyperstability, J. Mol. Biol., 400, 865-877, 2010

57.     Angkawidjaja C., Matsumura H., Koga Y., Takano K., Kanaya S., X-ray crystallographic and MD simulation studies on the mechanism of interfacial activation of a family I.3 lipase with two lids, J. Mol. Biol., 400, 82-95, 2010

58.     Foophow T., Tanaka S., Koga Y., Takano K., Kanaya S., Subtilisin-like serine protease from hyperthermophilic archaeon Thermococcus kodakaraensis with N- and C-terminal propeptides, Protein Eng. Des.Sel., 23, 347-355, 2010

59.     Takeuchi Y., Tanaka SI., Matsumura H., Koga Y., Takano K., Kanaya S., Requirement of a unique Ca(2+)-binding loop for folding of Tk-subtilisin from a hyperthermophilic archaeon, Biochemistry, 48, 10637-10643, 2009

60.     Tanaka SI., Matsumura H., Koga Y., Takano K., Kanaya S., Identification of the interactions critical for propeptide-catalyzed folding of Tk-Subtilisin, J. Mol. Biol., 394, 306-319, 2009

61.     Budiman C., Bando K., Angkawidjaja C., Koga Y., Takano K., Kanaya S., Engineering of monomeric FK506-binding protein 22 with peptidyl prolyl cis-trans isomerase. Importance of a V-shaped dimeric structure for binding to protein substrate, FEBS J., 276(15), 4091-4101, 2009

62.     Takano K., Higashi R., Okada J., Mukaiyama A., Tadokoro T., Koga Y., Kanaya S., Proline effect on the thermostability and slow unfolding of a hyperthermophilic protein, J. Biochem., 145(1), 79-85, 2009

63.     Rohman M. S., Tadokoro T., Angkawidjaja C., Abe Y., Matsumura H., Koga Y., Takano K., Kanaya S., Destabilization of psychrotrophic RNase HI in a localized fashion as revealed by mutational and X-ray crystallographic analyses, FEBS J., 276(2), 603-613, 2009

64.     Kuwahara K., Angkawidjaja C., Matsumura H., Koga Y., Takano K., Kanaya S., Importance of the Ca2+-binding sites in the N-catalytic domain of a family I.3 lipase for activity and stability, Protein Eng. Des. Sel., 21(12), 737-744, 2008

65.     Tanaka S., Takeuchi Y., Matsumura H., Koga Y., Takano K., Kanaya S., Crystal structure of Tk-subtilisin folded without propeptide: requirement of propeptide for acceleration of folding, FEBS Lett., 582, 3875-3878, 2008

66.     Rohman M. S., Koga Y., Takano K., Chon H., Crouch R. J., Kanaya S., Effect of the disease-causing mutations identified in human ribonuclease (RNase) H2 on the activities and stabilities of yeast RNase H2 and archaeal RNase HII, FEBS J., 275(19), 4836-4849, 2008

67.     Tadokoro T., Matsushita K., Abe Y., Rohman M. S., Koga Y., Takano K., Kanaya S., Remarkable stabilization of a psychrotrophic RNase HI by a combination of thermostabilizing mutations identified by the suppressor mutation method, Biochemistry, 47(31), 8040-8047, 2008

68.     Koga Y., Katsumi R., You D. J., Matsumura H., Takano K., Kanaya S., Crystal structure of highly thermostable glycerol kinase from a hyperthermophilic archaeon in a dimeric form, FEBS J., 275(10), 2632-2643, 2008

69.     Dong H., Mukaiyama A., Tadokoro T., Koga Y., Takano K., Kanaya S., Hydrophobic effect on the stability and folding of a hyperthermophilic protein, J. Mol. Biol., 378(1), 264-272, 2008

70.     Pulido M. A., Tanaka S., Sringiew C., You D. J., Matsumura H., Koga Y., Takano K., Kanaya S., Requirement of left-handed glycine residue for high stability of the Tk-subtilisin propeptide as revealed by mutational and crystallographic analyses, J. Mol. Biol., 374(5), 1359-1373, 2007

71.     Haruki M., Tanaka M., Motegi T., Tadokoro T., Koga Y., Takano K., Kanaya S., Structural and thermodynamic analyses of Escherichia coli RNase HI variant with quintuple thermostabilizing mutations, FEBS J., 274(22), 5815-5825, 2007

72.     You D.J., Chon H., Koga Y., Takano K., Kanaya S., Crystal Structure of Type 1 Ribonuclease H from Hyperthermophilic Archaeon Sulfolobus tokodaii: Role of Arginine 118 and C-Terminal Anchoring, Biochemistry, 46(41), 11494-11503, 2007

73.     You D. J., Fukuchi S., Nishikawa K., Koga Y., Takano K., Kanaya S., Protein thermostabilization requires a fine-tuned placement of surface charged residues, J. Biochem.(Tokyo), 142(4), 507-516, 2007

74.     Angkawidjaja C., You DJ., Matsumura H., Kuwahara K., Koga Y., Takano K., Kanaya S., Crystal structure of a family I.3 lipase from Pseudomonas sp. MIS38 in a closed conformation., FEBS Lett., 581(26), 5060-5064, 2007

75.     Tanaka S., Matsumura H., Koga Y., Takano K., Kanaya S., Four new crystal structures of Tk-subtilisin in unautoprocessed, autoprocessed and mature forms: insight into structural changes during maturation, J. Mol. Biol., 372(4), 1055-1069, 2007

76.     Tadokoro T., Chon H., Koga Y., Takano K., Kanaya S., Identification of the gene encoding a type 1 RNase H with an N-terminal double-stranded RNA binding domain from a psychrotrophic bacterium, FEBS J., 274(14), 3715-3127., 2007

77.     Takano K., Katagiri Y., Mukaiyama A., Chon H., Matsumura H., Koga Y., Kanaya S., Conformational contagion in a protein: structural properties of a chameleon sequence, Proteins, 68(3), 617-625, 2007

78.     Tadokoro T., You D. J., Abe Y., Chon H., Matsumura H., Koga Y., Takano K., Kanaya S., Structural, thermodynamic, and mutational analyses of a psychrotrophic RNase HI, Biochemistry, 46(25), 7460-7468, 2007

79.     Pulido M. A., Koga Y., Takano K., Kanaya S., Directed evolution of Tk-subtilisin from a hyperthermophilic archaeon: identification of a single amino acid substitution responsible for low-temperature adaptation, Protein. Eng. Des. Sel., 20(3), 143-153, 2007

80.     Mukaiyama A., Koga Y., Takano K., Kanaya S., Osmolyte effect on the stability and folding of a hyperthermophilic protein, Proteins, 71(1), 110-118, 2007

81.     Angkawidjaja C., You D. J., Matsumura H., Koga Y., Takano K., Kanaya S., Extracellular overproduction and preliminary crystallographic analysis of a family I.3 lipase, Acta Cryst. F., 63, 187-189, 2007

82.     Katsumi R., Koga Y., You D.J., Matsumura H., Takano K., Kanaya S., Crystallization and preliminary X-ray diffraction study of glycerol kinase from the hyperthermophilic archaeon Thermococcus kodakaraensis, Acta Cryst. Sect. F. Struct. Biol. Cryst. Commun., 63(Pt 2), 126-129, 2007

83.     Tanaka S., Saito K., Chon H., Matsumura H., Koga Y., Takano K., Kanaya S., Crystal structure of unautoprocessed precursor of subtilisin from a hyperthermophilic archaeon: evidence for Ca2+-induced folding, J. Biol. Chem., 282(11), 8246-8255, 2007

84.     Kashii M., Fujisawa R., Adachi H., Mori Y., Sasaki T., Koga Y., Takano K., Kanaya S., Matsumura H., Inoue T., Murakami S., Sugamoto K., Yoshikawa H., Application of femtosecond laser ablation for detaching grown protein crystals from glass capillary tube, J. Biosci. Bioeng., 102(4), 372-374, 2006

85.     Mukaiyama A., Haruki M., Ota M., Koga Y., Takano K., Kanaya S., A hyperthermophili protein acquires functio at the cost of stability, Biochemistry., 45, 12673-12679, 2006

86.     Tanaka S., Saito K., Chon H., Matsumura H., Koga Y., Takano K., Kanaya S., Crystallization and preliminary X-ray diffraction study of active-site mutant of pro-Tk-subtilisin from a hyperthermophilic archaeon, Acta. Cryst. F, 62, 902-905, 2006

87.     You D., Chon H., Koga Y., Takano K., Kanaya S., Crystallization and preliminary crystallographic analysis of type1 RNaseH from the hyperthermophilic archaeon Sulfolobus tokodaii 7, Acta. Cryst. F, 62, 781-784, 2006

88.     Kakinouchi K., Adachi H., Matsumura H., Inoue T., Murakami S., Mori Y., Koga Y., Takano K., Kanaya S., Effect of ultrasonic irradiation on protein crystallization, J. Cryst. Growth, 292, 437-440, 2006

89.     Angkawidjaja C., Kuwahara K., Omori K., Koga Y., Takano K., Kanaya S., Extracellular secretion of Escherichia coli alkaline phosphatase with a C-terminal tag by type　I secretion system: purification and biochemical characterization, Prot. Eng. Des. Screen., 19(7), 337-343, 2006

90.     Chon H., Tadokoro T., Ohtani N., Koga Y., Takano K., Kanaya S., Identification of RNaseHI from psycrotorophic bacterium, Shewanella sp. SIB1 as a high-activity type RNase H, FEBS J., 273, 2264-2275, 2006

91.     Cornista J., Koga Y., Takano K., Kanaya S., Amyloidogenecity and pitrilysin sensitivity of a lysine-free derivative of amyloid -peptide cleaved from a recombinant fusion protein, J. Biotechnol., 122, 186-197, 2006

92.     Chon H., Matsumura H., Koga Y., Takano K., Kanaya S., Crystal structure and structure-based mutational analyses of RNase HIII from Bacillus stearothermophilus: a new type 2 RNase H with TBP-like substrate-binding domain at the N-terminus, J. Mol. Biol., 356, 165-178, 2006

93.     Pulido M, Saito K., Tanaka S., Koga Y., Morikawa M., Takano K., Kanaya S., Ca2-Dependent Maturation of subtilisin from a hyperthermophilic archaeon, Thermococcus kodakaraensis: the propeptide is a potent inhibitor of the mature domain but is not required for its folding, Appl. Environ. Microbiol., 72(6), 4154-4162, 2006

94.     Takano K., Endo S., Mukaiyama A., Chon H., Matsumura H., Koga Y., Kanaya S., Structure of amyloid  fragments in aqueous environments, FEBS J., 273,150-158, 2006

95.     Chon H., Matsumura H., Koga Y., Takano K., Kanaya S., Crystal structure of a human kynurenine aminotransferase II homologue from Pyrococcus horikoshii OT3 at 2.20 angstrom resolution, Proteins, 61, 685-688, 2005

96.     Suzuki Y., Ohnmar Y. W., Koga Y., Takano K., Kanaya S., Binding analysis of a psychrotrophic FKBP22 to a folding intermediate of protein using surface plasmon resonance, FEBS Let., 579, 5781-5784, 2005

97.     Angkawidjaja C., Paul A., Koga Y., Takano K., Kanaya S., Importance of a repetitive nine-residue sequence motif for intracellular stability and functional structure of a family I.3 lipase, FEBS Let., 579, 4707-4712, 2005

98.     Kato R., Nakano H., Konishi H., Kato K., Koga Y., Yamane T., Kobayashi T., Honda H., Novel strategy for protein exploration: high-throughput screening assisted with fuzzy neural network, J. Mol. Biol., 351, 683-692, 2005

99.     Takeuchi K., Kitano H., Adachi H., Mori Y., Sasaki.T, Matsumura H., Inoue T., Murakami S., Doi M., Koga Y., Takano K., Kanaya S., Protein crystal growth using laser-processed seed crystals, Jap. J. Appl. Phys., 44(5A), 3177-3179, 2005

100.   Chon H., Matsumura H., Koga Y., Takano K., Kanaya S., Crystallization and preliminary X-ray diffraction study of thermostable RNaseHII from Bacillus stearothermophilus, Acta Cryst., F61, 293-295, 2005

101.   Chon H., Matsumura H., Shimizu S., Maeda N., Koga Y., Takano K., Kanaya S., Overproduction and preliminary crystallographic study of a human kynurenine aminotransferase II homologue from Pyrococcus horikosii OT3, Acta Cryst., F61, 319-322, 2005

102.   Koga Y., Kato K., Nakano H. Yamane T., Inverting enantioselectivity of Burkholderia cepacia KWI-56 lipase by combinatorialmutation and high-throughput screening using single-molecule PCR and in vitro expression, J. Mol. Biol., 331, 585-592, 2003

103.   Koga Y., Kobayashi K., Yang J., Nakano H., Yamane T., In vitro construction and screening of Burkhorderia cepacia lipase library by using single-molecule PCR and cell-free protein synthesis., J. Biosci. Bioeng., 94, 84-86, 2002

104.   Kannan Y., Koga Y., Inoue Y., Haruki M., Takagi M., Imanaka T., Morikawa M., Kanaya, S., Active subtilisin-like protease from a hyperthermophilic archaeon in a form with a putative prosequence, Appl. Environ. Microbiol., 67, 2445-2452, 2001

105.   Koga Y., Haruki M., Morikawa M., Kanaya S., Stabilities of chimeras of hyperthermophilic and mesophilic glycerol kinases constructed by DNA shuffling, J. Biosci. Bioeng., 91, 551-556, 2001

106.   Yang J., Koga Y., Nakano H., Yamane T., Modifying the chain length selectivity of the lipase from Burkholderia cepacia KWI-56 through in vitro combinatorial mutagenesis in the substrate binding site, Protein Eng., 15, 147-152, 2001

107.   Haruki M., Hayashi K., Kouchi T., Muroya A., Koga Y., Morikawa M., Imanaka T., Kanaya S., Cloning of a ribonuclease HII gene from a hyperthermophilic archaeon: Purification and characterization of a recombinant enzyme, J. Bacteriol., 180, 6207-6214, 1998

108.   Koga Y., Morikawa M., Haruki M., Nakamura H., Imanaka T., Kanaya S., Thermostable glycerol kinase from a hyperthermophilic archaeon: Cloning of gene, overexpression in E.coli, and characterization of the enzyme, Protein Eng., 11, 1219-1227, 1998

 

【著書】

1.       Uehara R., Takano K., Kanaya S., Koga Y., “Hyperthermophilic subtilisin-like protease from Themococcus kodakarensis.” （分担執筆）“Biotechnology of Microbial Enzymes, production, biocatalysis and industrial applications” （Brahmachari　G., Demain A., Adrio A. 編）,　Academic Press, 81-117, 2017年

2.       古賀雄一,第4章”産業バイオテクノロジー”

（分担翻訳）ビジュアルバイオテクノロジー, 監訳者：福井希一, 内山　進, 松田史生, 化学同人, 73 - 92, 2017年

3.       古賀雄一　”微生物の利用事例紹介～森の中の微生物　柚子由来酵母の単離～”（分担執筆）”地方創生に関わる生物工学のとりくみ地域生物資源産業化事例集”（古賀雄一, 櫻谷英治,大政健史　編）三恵社, 128 -129, 2016年

4.       荒西睦雄, 古賀雄一”バイオ消毒剤,洗浄剤(酵素洗浄剤の応用)”（分担執筆）”食と健康の高安全化　－殺菌,滅菌,消毒,不活化,有害物質除去技術―”（作道章一　編著）,  S&T出版, 177 - 185, 2012年

5.       古賀雄一,”Enzymes in extreme environment (極限環境の酵素)”Part 1 Chapter 2（分担執筆）実践・化学英語リスニング　生化学編, 編集 福井希一, 福住俊一, ルーク・上田・サーソン,化学同人, 9 - 5, 2011年

6.       古賀雄一, “Metalloproteins in extreme environments （極限環境の金属タンパク質）”Part 3 Chapter 6,（分担執筆）実践・化学英語リスニング　生化学編, 編集 福井希一, 福住俊一, ルーク・上田・サーソン, 化学同人, 140 – 144, 2011年

7.       古賀雄一, 中野秀雄”酵素の機能改変における結晶構造の役割”（分担執筆）”タンパク質結晶の新展開, 監修 高野和文, シーエムシー出版, 251-261, 2008年

8.       Koga Y., Yamane T., Nakano H., “Creation of novel enantioselective lipases by SIMPLEX”, （分担執筆）In vitro transcription and translatio protocols, 2nd edition, Methods in molecular biology, Grandi G. 編, Humana Press, New Jersey, 165-181, 2007年

9.       中野秀雄・古賀雄一 “無細胞タンパク質合成系によるタンパク質工学”（分担執筆）”コンビナトリアル・バイオエンジニアリング”, 編集 植田充美, 近藤昭彦, 化学同人, 145-149,  2003 年

【国内招待講演（すべて口頭発表）】

1. 古賀雄一, “生物資源を活用したグローカルバイオ研究”, グローカルバイオシンポジウム“バイオ民主化時代の到来と地域発イノベーションの加速”, 日本橋ライフサイエンスビルディング, 東京, 2019年3月23日

2. 古賀雄一, “地域生物資源から新しい価値を創る,グローカルバイオテクノロジー”, 日本生物工学会北日本支部シンポジウム, 北海道大学, 札幌市, 2018年10月15日

3. 古賀雄一,”超好熱菌由来酵素の構造機能相関研究とその応用”, 第69回日本生物工学会年次大会3AAp01, 早稲田大学, 東京, 2017年9月13日

4. 古賀雄一,”極めて安定性の高いプロテアーゼの生産と活用”, 大阪大学EDGE CONNECT ×SUNTORY, サントリーワールドリサーチセンター, 精華町, 2016年12月16日

5. 古賀雄一,　”微生物の利用事例紹介  超好熱菌の利用と柚子由来酵母の単離”,　第1回日本生物工学会グローカルバイオ研究部会講演会,　徳島大学,　徳島市, 2016年11月5日

6. 古賀雄一, “プリオン分解が可能な医療用洗浄剤を目指した極めて熱安定性の高いプロテアーゼの実用化”次世代医療システム産業化フォーラム, 大阪商工会議所, 大阪市, 2013年9月11日

7. 古賀雄一, “好熱性プロテアーゼによるプリオン不活化”プラズマ照射による医療用品の滅菌エンドトキシンならびにプリオン不活化法と応用に関する研究開発専門委員会, 千里ライフサイエンスセンター, 豊中市, ,2011年3月2日

8. 古賀雄一, “Analysis of mechanism for enantioselectivity of lipase from Burkholderia cepacia KWI-56 “北海道大学大学院地球環境科学研究院セミナー, 北海道大学, 札幌市, 2010年6月15日

9. 古賀雄一, ““極限環境”の生命を支えるタンパク質の利用－世界一タフな酵素を使ったバイオ洗浄剤”千里ライフサイエンスセンター市民セミナー, 千里ライフサイエンスセンター、豊中市, 2010年3月22日

 

【国内学会発表（口頭発表および、研究発表）】（総数114回のうち過去5年間を記述）

1. Ryo Uehara, Nanako Dan, Hiroshi Amesaka, Takuya Yoshizawa, Yuichi Koga, Shigenori Kanaya, Kazufumi Takano, Hiroyoshi Matsumura, “Insertion loop-mediated folding propagation of Tk-subtilisin, which governs efficient maturation in high-temperature environments”, 第22回日本蛋白質科学会, つくば国際会議場, 2022年6月7-9日

2. Chukuwebuka Maxwell Ononugbo, Masahide Nagao, Hidekazu Kishi, NorikoYamano-Adachi, Yuichi Koga, Takeshi Omasa, “Engineering the beta-sandwich domain 1 of a hyperthermophile for  improved soluble expression for novel binding”, 第22回日本蛋白質科学会, つくば国際会議場, 2022年6月7-9日

3. 古賀雄一，外尾竜太、“超好熱古細菌（始原菌）のGlycerol kinaseの分子制御メカニズム”

4. 33回 日本Archaea研究会講演会、オンライン開催,

5. 兼吉航平, 黒田昂輝, 山野-足立範子, 鬼塚正義, 古賀雄一, 内山圭司, 大政健史, “生産性向上を目指した医薬品抗体生産細胞における分泌過程解析”, 化学工学会第84回年会, 芝浦工業大学, 東京, 2019年3月13 - 15日

6. 岡本奈子, 野村嘉紀, 有永幹基, 鬼塚正義, 山野範子, 古賀雄一, 大政健史, “Chinese hamster ovary細胞を宿主としたサメ由来重鎖抗体分泌生産の試み”, 化学工学会第50回秋季大会, 鹿児島大学, 鹿児島市, 2018年9月18-20日

7. 山野範子, 隈元信貴, 中西悠人, 吉冨耕太, 古賀雄一, 大政健史, ”組換えタンパク質生産のための安定染色体への遺伝子ターゲティング”, 化学工学会第50回秋季大会, 鹿児島大学, 鹿児島市, 2018年9月18-20日

8. 兼吉航平, 山野範子, 古賀雄一, 鬼塚正義, 内山圭司, 大政健史, ”高生産株の構築を目指した蛍光標識抗体による細胞内分泌過程解析”, 生物工学若手研究者の集い（若手会）夏のセミナー, 北見工業大学, 北見市, 2018年6月30日-7月1日

9. 鎌田祥平, 鬼塚正義, 黄川田隆洋, 古賀雄一, 山野範子, 大政健史, ”トレハローストランスポーター発現CHO細胞を用いたトレハロース含有培地における高凝集性抗体の凝集抑制”, 化学工学会第83回年会, 関西大学, 吹田市, 2018年3月13-15日

10. 来住秀憲, 大政健史, 山野範子, 古賀雄一, “超好熱菌由来酵素の基質結合に関するドメインに基づいた新規標的結合分子の開発”, 第70回日本生物工学会年次大会, 2Ip04, 関西大学, 吹田市, 2018年9月5-7日

11. 巽　祐介, 足立航平, 山野範子, 大政健史, 古賀雄一, “Split intinのprotein trans-splicingを利用した耐熱性プロテアーゼ再構成の検討”, 第70回日本生物工学会年次大会, 3Ma05, 関西大学, 吹田市, 2018年9月5-7日

12. 宮田大樹, 大政健史, 山野範子, 古賀雄一,“Tk-subtilisinの有機溶媒耐性の解析”, 第70回日本生物工学会年次大会, 3Ma06, 関西大学, 吹田市, 2018年9月5-7日

13. 北　一真, 山野範子, 古賀雄一, 大政健史, “長期的に安定な組換えタンパク質高生産株の構築に向けたCHO細胞における染色体多様性の解析”, 第70回日本生物工学会年次大会, 3Oa04, 関西大学, 吹田市, 2018年9月5-7日

14. 兼吉航平, 内山圭司,  鬼塚正義,  山野範子,  古賀雄一,  大政健史, “高生産株の構築を目指した抗体生産CHO細胞内の分泌過程解析”, 第69回日本生物工学会年次大会 3PJ202, 早稲田大学, 東京, 2017年9月11-14日

15. 来住秀憲, 杉田慎之介, 大政健史, 古賀雄一, ”βサンドイッチドメインを用いた結合分子骨格の検討”, 第17回日本蛋白質科学会年会 3P130,  仙台国際センター, 仙台市, 2017年6月20-2日

16. 田口萌恵, 大政健史, 古賀雄一, ”超好熱古細菌由来プロテアーゼTk0076の成熟化機構解明を目指したプロペプチドの探索”, 第17回日本蛋白質科学会年会 3P090,  仙台国際センター, 仙台市, 2017年6月20-22日

17. 古賀雄一, 清水七海, 足立航平, “超好熱菌由来Thermococcus kodakarensis  KOD1 由来subtilisin 様プロテアーゼとその利用”, 日本農芸化学会2017年大会 2J30p01, 京都女子大学, 京都市, 2017年 3月17-20日

18. 杉田慎之介,  大政健史,  古賀雄一, ”ケラチン分解酵素の機能未知βサンドイッチドメインの機能解析”, 日本農芸化学会2017年大会 2J30p02,  京都女子大学, 京都市,  2017年 3月17-20日

19. 古賀雄一, ”PrPScの不活化が可能な酵素洗浄剤開発”, 28年度厚生労働省科学研究費難治性疾患克服事業プリオン病のサーベイランスと感染予防に関する調査研究班合同報告会, アルカディア市ヶ谷, 東京, 2017年1月16-17日

20. 古賀雄一, Extremophiles and applications of their unique enzymes,  Sakura Scienceプログラム, 大阪大学国際交流センター, 吹田市, 2016年10月24日

21. 古賀雄一, 清水七海, 足立航平, 大政健史, ”超好熱菌由来Thermococcus kodakarensis KOD1 由来subtilisin 様プロテアーゼとその利用”, 第68回日本生物工学会大会, 富山国際会議場, 富山市,  2016年9月28-30日

22. 杉田慎之介, 大政健史, 古賀雄一, ”ケラチン分解酵素の機能不明C末端ドメインの機能解析”, 第68回日本生物工学会大会　1P1p031, 富山国際会議場, 富山市, 2016年9月28-30日

23. 古賀雄一, 高野　築, 小西佳菜子, 小林　淳, 大政健史, ”超好熱古細菌由来glycerol代謝酵素の酵素活性調整機構”, 第16回日本蛋白質科学会年会　2P046, 福岡国際会議場, 福岡市, 2016年6月7-9日

24. 足立航平, 上原了, Tannous E. Antonios, 大政健史,  古賀雄一, “Iintein を利用した超好熱菌由来プロテアーゼ（Tk-SP）のインテインを用いたの発現系の構築” 日本農芸化学会2016年大会 3D034, 札幌コンベンションセンター, 札幌市, 2016年3月27-30日

25. 古賀雄一, “PrPScの不活化が可能な酵素洗浄剤開発”,  27年度厚生労働省科学研究費難治性疾患克服事業プリオン病のサーベイランスと感染予防に関する調査研究班合同報告会, アルカディア市ヶ谷, 東京, 2016年1月18-19日

 

【国外招待講演（口頭発表）】

1. Koga Y., “Protein engineering on a Subtilisin-like protease from a hyper-thermophilic archaeon and its application”, 2019 Sakura-Bio Meeting, Nagoya, Japan, 2019 March 30-31

2. Koga Y., “Protein engineering of subtilisin-like protease from a hyper-thermophilic archaeon”, 2018 KSBB Spring Meeting and International Symposium A101, Yeosu, Korea, 2018 Apr 19-20

3. Koga Y., “Protein engineering on a subtilisin-like protease from a hyper-thermophilic archaeon”, The 29th Annual meeting of the Thai society for biotechnology and international conference EE-O-003, Bangkok, Thailand, 2017 Nov23-25

4. Koga Y., “Subtilisin-like proteases from a hyper-thermophilic archaeon and their application”, BIOTEC-OSAKA Univ. Special Seminar, Bangkok, Thailand, 2017 Aug 3

 

【国外発表（研究発表）】

1. Kuroda K., Kaneyoshi K., Yamano N., Koga Y., Omasa T., ”Quantitative analysis of intracellular secretion of recombinant antibodies in CHO cells for establishment of high producers”, The 30th annual meeting of the Thai society for biotechnology and international conference, Bangkok, Thailand, 2018 Nov 22-23

2. Kita K., Yamano N., Koga Y., Omasa T., “Analysis of chromosomal diversity in CHO cell lines for establishment of stable highly-productie cell line in recombinant therapeutic production”, YABEC2018, Taipei, Taiwan, 2018 Nov 15-17

3. Quach C., Frank J., Yamano N., Koga Y., Omasa T., “Evaluation of function and activity of mutant p53 in Cricetulus griseus cell lines” P-29, JAACT2018, Tsukuba, Japan, 2018 Nov 5-8

4. Kuroda K., Kaneyoshi K., Yamano N., Koga Y., Omasa T., ”Quantitative analysis of secretion process in recombinant CHO cells producing ”sifficult-to express” IgG”, P-025, JAACT2018, Tsukuba, Japan, 2018 Nov 5-8

5. Kaneyoshi K., Kuroda K., Yamano N., Koga Y., Omasa T., “Analysis of intracellular secretion processes by citrine fusion IgG aiming to establish high producer CHO cells”, P-014, JAACT2018, Tsukuba, Japan, 2018 November 5-8

6. Koga Y., Uehara R, Takano K., “Hyper-thermophilic subtilisin-like proteases from Thermococcus kodakarensis and their application”, Extremophiles 2016  P174, Kyoto, Japan, 2016 Sep 12-16

7. Maegawa D., Adachi K., Omasa T., Koga Y., “Construction of a novel expression system of toxic protease froma hyperthermophilic archaeon Thermococcus kodakarensis KOD1”, Extremophiles 2016 P173, Kyoto, Japan, 2016 Sep 12-16

8. Koga Y., Shimizu N., Sakudo, A., Kanaya S., “Proteolysis of abnormal prion protein with a thermostable protease from a hyper-thermophilic archaeon Thermococcus kodakarensis KOD1”, Biophysical society 59th annual meeting  2691, Baltimore MA, USA, 2015 Feb 7-11

9. Okano H., KanayaE., Ozaki M.,Angkwaidjaja C., Koga Y., Kanaya S., “Crystal structure of glycoside hydrolase family 9 endoglucanase with an N-terminal Ig-like domain isolated from leaf-branch compost”, Biophysical society 59th annual meeting 1132, Baltimore MA, USA, 2015 Feb 7-11

10. Okano K., Sulaiman S., Kanaya E., You D. J., Koga Y., Kanaya S., “Characterization of novel metagenome-derived esterase and cutinase from leaf- branch compost”, 5th Congress of the European Microbiologists FEMS 2013, Leipzig, Germany, 2013 July 21-25

11. Osaki M., Kanaya E., Okano H., Koga Y., Kanaya S., “Isolation of novel cellulolytic enzymes from leaf-branch compost by a metagenomic approach”, 5th Congress of the European Microbiologists FEMS 2013, Leipzig, Germany, 2013 July 21-25

12. You D. J., Jongruja N., Tannous E., Angkawidjaja C.,Jung H. S., Koga Y., Kanaya S., “Crystal structure and salt-dependent folding of ribonuclease H1 from halophilic archaeon Halobacterium salinarum NRC-1”, Nagoya Symposium - Frontiers in structural physiology on the occasion of the inauguration of the cellular and structural physiology institute and the NEDO project at Nagoya University, Nagoya, Japan, 2013 Jan 22-24

13. Takano K., Okada J., Koga Y., Kanaya S., “Slow unfolding pathway of a hyperthermophilic protein, Tk-RNase H2, examined by pulse proteolysis using a super-stable protease, Tk-subtilisin”, 9th International congress on extremophiles, Sevilla Spain, 2012 Sep 10-13

14. Koga Y., Sakudo A., “Proteolysis of PrPSc with a thermostable protease and the analysis of its infectivity, Asian Pacific Prion Symposium 2012 P2-19, Yokohama, Japan, 2012 Jul 29-30

15. You D. J., Yokoyama K., Jongruja N., Angkawidjaja C., Koga Y., Kanaya S.,“Crystal  Structure  and  Salt-dependent  Folding  of  Ribonuclease H1 from Halophilic Archaeon Halobacterium salinarum NRC-1”The Korean society for microbiology and biotechnology 2012 international symposium & annual meeting H-24, Busan, Korea, 2012 Jun 27-29

16. Jongruja N., You D. J., Angkwaidjaja C., Yokoyama K., Tannous E., Kanaya E., Koga Y., Kanaya S., “Structures of RNase H3 from Aquifex aeolicus and RNase H1 from Halobacterium salinarum NRC-1”, RNase H 2012, Edinburgh, Scotland, 2012 Sep 5-7

17. Shikakura, T., Koga Y., Abe M., Seino S., Nakagawa T., Kanaya S., “Development activation technology of immobilized enzyme using hyperthermia”, KAIST-Osaka University symposium, Daejon, Korea, 2012 Aug 6-8

18. Uehara R., Koga Y., Takano K., Kanaya S., “Accelerated Maturation of Tk-subtilisin by the Mutation at the C-terminus of Propeptide”, The 11th Global COE International Symposium, Suita , Japan, 2011 Dec 19-21

19. Koga Y., Hokao R., Takano K, Kanaya S., Nakano H., “Studies on enantioselectivity of lipase from Burkholderia cepacia KWI-56, Enzyme Engineering XXI P38, Vail Co, USA, 2011 Sep18-22

20. Uehara R., Koga Y., Takano K., Kanaya S., “Mechanism for accelerating maturation of pro-Tk-subtilisin with the mutation at the C-terminus of propeptide”, Archen-Osaka Mini Symposium, Archen, Germany, 2011 Sep 1

21. Koga Y., Sakudo A., “Enzymatic degradation of PrPSC by a hyperthermostable protease”, Asian Pacific Prion Symposium 2011 P2-17, Karuizawa, Japan, 2011 Jul 10-11

22. Yamato S., Kanaya E., Sulaiman S., Kim J. J., Koga Y., Takano K., Kanaya S., “Isolation of a bacterial cutinase homolog from leaf-branch compost using metagenomic approach”, 4th Congress of the European Microbiologists FEMS 2011, Geneva, Switzerland, 2011 June 26-30

23. Koga Y., Uehara R., Fukuda H., Takano K., Kanaya S., “Thermodynamic analysis of enantioselectivity of mutant lipases”, The Eleventh China-Japan-Korea Joint Symposium on Enzyme Engineering P-34, Chengdu, China, 2010 Nov.5-8

24. Okada J., Okamoto T., Mukaiyama A., Tadokoro T., You D.J. Chon H., Koga Y., Takano K., Kanaya S., “Evolution and Thermodynamics for Slow Unfolding of Hyperstable Proteins”, 8th International Congress on Extremophiles, Ponta Delgada, Portugal, 2010 Sep 12-16

25. Koga Y., Tanaka S., Sakudo A., Ikuta K., Takano K., Kanaya S., “Degradation of abnormal prion protein by a new protease from a hyperthermophile”, Prion 2010 PP08-29, Salzburg, Austria, 2010 Sep 8-11

26. Koga Y., Uehara R., Fukuda H., Takano K., Kanaya S., “Studies on enantioselectivity of lipase from Burkholderia cepacia KWI-56”, BIOCAT 2010, Hamburg, Germany, 2010 Aug 29- Sep 2

27. Hokao R., Koga Y., Takano K., Kanaya S., “Structural and functional characterization of glycerol kinase from the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1”, Asia Pacific Biochemical Engineering Conference 2009, Kobe, Japan, 2009 Nov 24-28

28. Koga Y., Katsumi R., You D.J., Matsumura H., Takano K., Kanaya S., “The Role of C-terminal region in stability of glycerol kinase from Thermococcus kodakarensis KOD1”, The 21rd Symposium of the Protein Society, 551, Boston MA, USA, 2007 20-25

29. Rohman S. M., Koga Y., Takano K., Kanaya S., “Gene cloning and characterization of psychrophile RNase III”, International Symposium on industrial enzymes, P8, Ikeda, 2006 Feb 15-16

30. Angkawidjaja C., Kuwahara K., Koga Y., Takano K., Omori K., Kanaya S., “ Extracellular secretion of alkaline phosphatase by a type I secretion system”,  International Symposium on industrial enzymes  P7, Ikeda, 2006 Feb 15-16

31. You D. J., Fukuchi S., Nishikawa K., Koga Y., Takano K., Kanaya S., “Effect of the introduction of surface charged residues on the thermostability of E.coli RNase HI”, The 3rd open workshop: chemistry of biological process created by water ans biomolecules P110, Okazaki, Japan, 2006 Jan 6-7

32. Pulido A., Koga Y., Takano K., Kanaya S., “Improved maturation efficiency at low temperature of a hyperthermophilic subtilisin by in vitro random mutagenesis”, The 3rd open workshop: chemistry of biological process created by water ans biomolecules, P109, Okazaki, Japan, 2006 Jan 6-7

33. Tadokoro T., Koga Y., Takano K., Kanaya S., “Biochemical properties of RNase HI from Shewanella oneidensis MR-1”, The 3rd open workshop: chemistry of biological process created by water ans biomolecules, P108, Okazaki, Japan, 2006 Jan 6-7

34. Angkawidjaja C., Paul A., Koga Y., Takano K., Omori K., Kanaya S., “Importance of a repetitive nine-residue sequence motif for intracellular stability and functional structure of a family I.3 lipase” The 3rd open workshop: chemistry of biological process created by water ans biomolecules, P107, Okazaki, Japan, 2006 Jan 6-7

35. Tanaka SI., Saito K., Koga Y., Takano K., Kanaya S., “Analysis of the maturation process of subtilisin from Thermococcus kodakarensis KOD1 using active-site mutants”, International symposium on extremophiles and their applications P-13, Tokyo, Japan, 2005 Nov 29 - Dec 2

36. Koga Y., Nakano H., Yamane T., “In vitro screening of Burkholderia cepacia KWI-56 lipase library by using single-molecule PCR and cell-free protein synthesis: improving the enantioselectivity of the lipase”, Enzyme Engineering XYII  15, Santa Fe, USA, 2003 Nov 9-14

37. Koga Y., Yang H., Nakano H., Yamane T., “In vitro screening of Burkhorderia cepaciaKWI-56 lipase library by using single-molecule PCR and cell-free protein synthesis: improbing the enantioselectivity of the lipase”, BIOCAT 2002 P010, Humburg Germany, 2002 Jul 28-31